충북대학교 종양연구소에서 다음과 같이 초청세미나를 개최하오니 많은 참여 부탁드립니다.
- 다 음 -
1. 연 자 : 전 상 용 교수 (KAIST)
2. 일 시 : 2019년 10월 15일 (화) 오후 4시
3. 연 제 : Small Lipid Nanoparticles as a Systemic siRNA Delivery Platform
4. 장 소 : 종양연구소 세미나실 104호
5. 발 표 내 용
Small Lipid Nanoparticles as a Systemic siRNA Delivery Platform
Efforts to develop a new nanocarrier for RNAi-based cancer therapy led to our development of nontoxic SLNPs, which resulted in the systemic delivery of siRNA and significantly inhibited tumor growth in vivo. A new cationic cholesterol derivative, MA-Chol, was able to form complexes with siRNA with high efficiency, resulting in the formation of SLNPs with a neutral surface charge. Furthermore, as MA-Chol, unlike DOTAP and DC-Chol, was designed to generate two nontoxic endogenous compounds, arginine and cholesterol, upon degradation in vivo, the resulting SLNPs showed little cytotoxicity, both in vitro and in vivo, even at relatively high concentrations. The siRNA-loaded SLNPs (siKSP@SLNPs) showed a high level of target gene silencing in several cancer cell lines. Furthermore, systemically injected siKSP@SLNPs showed preferential accumulation at the tumor site and dose-dependently inhibited tumor growth in a prostate cancer model. Taken together, these findings indicate that these cationic cholesterol derivative-based SLNPs possess many of the attributes required of a delivery platform for RNAi-based cancer therapy, including small size, lack of toxicity, neutral surface charge, and highly efficient complex formation. This SLNP platform may therefore be applicable to RNAi-based treatment of cancers and other hard-to-cure diseases.